Recombinant proteins are widely used as reagents in laboratory experiments and to generate antibody probes for examining protein synthesis within cells and organisms.Recombinant DNA is used to identify, map and sequence genes, and to determine their function.The most common application of recombinant DNA is in basic research, in which the technology is important to most current work in the biological and biomedical sciences.Recombinant DNA is widely used in biotechnology, medicine and research.When pst1 RE is used it knock out Ampicillin resistant gene from the plasmid, so that the recombinant cell become sensitive to Ampicillin.Īpplication of Recombinant DNA technology ![]() For examples, PBR322 plasmid vector contains different marker gene (Ampicillin resistant gene and Tetracycline resistant gene.For isolation of recombinant cell from non-recombinant cell, marker gene of plasmid vector is employed.The selection process involves filtering the transformed host cells only. ![]()
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